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KMID : 0360220110520050618
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Journal of the Korean Ophthalmological Society
2011 Volume.52 No. 5 p.618 ~ p.623
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Effect of Creatine on the Survival of RGC-5 Cells under Serum Deprivation
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Kim Jae-Woo
Hong Jung-Heum
Kang Sun-Hee
Kim Yun-Young
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Abstract
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Purpose: To evaluate the protective effect of creatine on the survival of retinal ganglion cells after serum deprivation.
Methods: RGC-5 cells were exposed to 5 mM creatine with serum-free media for 4 days. Cellular survival and mitochondrial respiratory activity were measured with MTT assay and resazurin assay, respectively. Degree of apoptosis was evaluated with vital staining using acridine orange/Hoechest 33342 and flow cytometric analysis using annexin/PI, respectively.
Results: Creatine increased cellular survival of RGC-5 cells significantly after serum deprivation. Additionally, creatine increased mitochondrial respiratory activity and inhibited apoptosis of RGC-5 cells.
Conclusions: The energy precursor creatine increased survival of retinal ganglion cells after serum deprivation. Creatine could be relevant for the cytoprotection of retinal ganglion cells.
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KEYWORD
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Creatine, Cytoprotection, Retinal ganglion cell
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